2026-03-16T00:11:34Z https://ehime-u.repo.nii.ac.jp/oai

oai:ehime-u.repo.nii.ac.jp:02002274 2023-10-13T05:26:06Z 664

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes TAKENOUCHI, Satoshi Faculty of Agriculture, Ehime University SUGAHARA, Takuya Faculty of Agriculture, Ehime University 464 491.4 hybridoma immunoglobulin production lactate dehydrogenase peripheral blood lymphocytes serum-free culture immunostimulation IgM Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 μg/ml under serum-free condition. LDHs from pig muscle and pig heart also accelerated IgM production 8.4- and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied with activation of cell proliferation. LDH from rabbit muscle facilitated IgM and IgG production by human peripheral blood lymphocytes. This means LDH stimulates immunoglobulin production not only by the specified hybridoma cell line, but also by unspecified immunoglobulin producers. LDH from rabbit muscle enhanced IgM production of transcription-suppressed HB4C5 cells treated with actinomycin D. The IPSF effect of LDH was slightly weakened by sodium fluoride (translation inhibitor) treatment of HB4C5. Moreover, the amount of intracellular IgM of monensin-treated HB4C5 cells was obviously enhanced by LDH. This result means that the IPSF effect of LDH is irrelevant to the post-translation activity of target cells. It is expected from these findings that LDH from rabbit muscle accelerates the translation step to enhance immunoglobulin productivity. The immunoglobulin production stimulating activity of LDH was inhibited by colchicine, endocytosis inhibitor. This fact suggests that it is necessary for LDH to be taken by target cells for acting as IPSF. Springer 2003-09 eng journal article AM https://ehime-u.repo.nii.ac.jp/records/2002274 19002935 10.1023/B:CYTO.0000015838.06536.de AA10678299 0920-9069 Cytotechnology 42 3 133 143 https://ehime-u.repo.nii.ac.jp/record/2002274/files/AA10678299_42_133-143.pdf application/pdf