| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-19 |
| タイトル |
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|
タイトル |
Degranulation of RBL-2H3 rat basophilic leukemia cells is synergistically inhibited by combined treatment with nobiletin and lactoferrin |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nobiletin |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
lactoferrin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
RBL-2H3 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
degranulation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
anti-allergic effect |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
MIYAKE, Kaho
| en |
MIYAKE, Kaho
Graduate School of Bioscience and Biotechnology, Chubu University
|
Search repository
TANAKA, Mamoru
| en |
TANAKA, Mamoru
Graduate School of Bioscience and Biotechnology, Chubu University
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Search repository
YOKOYAMA, Sayaka
| en |
YOKOYAMA, Sayaka
Department of Food and Nutritional Environment, Kinjo Gakuin University
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Search repository
RUI, Lu
| en |
RUI, Lu
Graduate School of Bioscience and Biotechnology, Chubu University
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Search repository
KOIDA, Ayaka
| en |
KOIDA, Ayaka
Faculty of Health and Medical Sciences, Aichi Shukutoku University
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Search repository
KOZAI, Hana
| en |
KOZAI, Hana
Graduate School of Bioscience and Biotechnology, Chubu University
|
Search repository
OKAMOTO, Takeaki
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The aim of this study was to elucidate the anti-allergic effects of polymethoxyflavonoids in combination with milk proteins and the mechanism of inhibition. Three polymethoxyflavonoids and two milk proteins were exposed to the rat basophilic leukemia cell line RBL-2H3. β-hexosaminidase was used as an indicator of degranulation inhibition. The mechanism of inhibition was examined by measuring intracellular Ca2+ levels and western blot method. In the degranulation inhibition test with polymethoxyflavonoids and milk proteins alone, nobiletin was the strongest inhibitor in the polymethoxyflavonoid group and lactoferrin in the milk protein group. Next, co-stimulation with nobiletin and lactoferrin showed stronger synergistic degranulation inhibition than treatment with nobiletin or lactoferrin alone. Western blot analysis showed that co-stimulation with nobiletin and lactoferrin significantly downregulated the induction of phospholipase Cγ 1 phosphorylation. The degranulation response in RBL-2H3 cells was synergistically suppressed by co-stimulation of nobiletin and lactoferrin acting on both Ca2+-dependent and Ca2+-independent pathways. |
|
言語 |
en |
| 書誌情報 |
en : Cytotechnology
巻 76,
号 3,
p. 341-349,
発行日 2024-06
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| 出版者 |
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|
出版者 |
Springer Nature |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0920-9069 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10678299 |
| PubMed番号 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
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関連識別子 |
38736731 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1007/s10616-024-00625-2 |
| 権利 |
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|
権利情報 |
© 2024 Springer Nature B.V. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s10616-024-00625-2 |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Accepted Manuscript_AA10678299_76_341-349.pdf (659 KB)
