| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-19 |
| タイトル |
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|
タイトル |
Experimental interventions attenuate a conjunctival epidermal metaplasia model |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
conjunctiva |
| キーワード |
|
|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
keratinization |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
epidermal metaplasia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PAX6 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glucocorticoid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
serum application |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
TAKEZAWA, Yuki
| en |
TAKEZAWA, Yuki
Department of Ophthalmology, Graduate School of Medicine, Ehime University
|
Search repository
KAMON, Masayoshi
| en |
KAMON, Masayoshi
Department of Developmental Biology and Functional Genomics, Graduate School of Medicine, Ehime University
|
Search repository
HIRAKI-KAMON, Keiko
| en |
HIRAKI-KAMON, Keiko
Department of Developmental Biology and Functional Genomics, Graduate School of Medicine, Ehime University
|
Search repository
MITANI, Arisa
| en |
MITANI, Arisa
Department of Ophthalmology, Graduate School of Medicine, Ehime University
|
Search repository
SHIRAISHI, Atsushi
| en |
SHIRAISHI, Atsushi
Department of Ophthalmology, Graduate School of Medicine, Ehime University
|
Search repository
KATO, Hidemasa
| en |
KATO, Hidemasa
Department of Developmental Biology and Functional Genomics, Graduate School of Medicine, Ehime University
|
Search repository
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The conjunctiva is a non-keratinized, stratified columnar epithelium with characteristics different from the cornea and eyelid epidermis. From development to adulthood, a distinguishing feature of ocular versus epidermal epithelia is the expression of the master regulator PAX6. A conditionally immortalized conjunctival epithelial cell line (iHCjEC) devoid of stromal or immune cells established in our laboratory spontaneously manifested epidermal metaplasia and upregulated expression of the keratinization-related genes SPRR1A/B and the epidermal cytokeratins KRT1 and KRT10 at the expense of the conjunctival trait. In addition, iHCjEC indicated a significant decrease in PAX6 expression. Dry eye syndrome (DES) and severe ocular surface diseases, such as Sjögren’s syndrome and Stevens-Johnson syndrome, cause the keratinization of the entire ocular surface epithelia. We used iHCjECs as a conjunctiva epidermal metaplasia model to test PAX6, serum, and glucocorticoid interventions. Reintroducing PAX6 to iHCjECs resulted in upregulating genes related to cell adhesion and tight junctions, including MIR200CHG and CLDN1. The administration of glucocorticoids or serum resulted in the downregulation of epidermal genes (DSG1, SPRR1A/B, and KRT1) and partially corrected epidermal metaplasia. Our results using an isolated conjunctival epidermal metaplasia model point toward the possibility of rationally “repurposing” clinical interventions, such as glucocorticoid, serum, or PAX6 administration, for treating epidermal metaplasia of the conjunctiva. |
|
言語 |
en |
| 書誌情報 |
en : Experimental Eye Research
巻 243,
p. 109916,
発行日 2024-06
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| 出版者 |
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出版者 |
Elsevier Ltd |
|
言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
PISSN |
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収録物識別子 |
0014-4835 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00641055 |
| PubMed番号 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
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|
関連識別子 |
38679224 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.exer.2024.109916 |
| 権利 |
|
|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2024 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Accepted Manuscript_AA00641055_243_109916.pdf (13.1 MB)
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Supplementary_Table.zip (113 KB)
