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Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes
https://ehime-u.repo.nii.ac.jp/records/2002274
https://ehime-u.repo.nii.ac.jp/records/2002274cdfb4bfb-4d66-4efd-b596-d6de1ab1e6ad
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA10678299_42_133-143.pdf
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2012-02-17 | |||||||||
| タイトル | ||||||||||
| タイトル | Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | hybridoma | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | immunoglobulin production | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | lactate dehydrogenase | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | peripheral blood lymphocytes | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | serum-free culture | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | immunostimulation | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | IgM | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
TAKENOUCHI, Satoshi
× TAKENOUCHI, Satoshi
× SUGAHARA, Takuya
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| 抄録 | ||||||||||
| 内容記述 | Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 μg/ml under serum-free condition. LDHs from pig muscle and pig heart also accelerated IgM production 8.4- and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied with activation of cell proliferation. LDH from rabbit muscle facilitated IgM and IgG production by human peripheral blood lymphocytes. This means LDH stimulates immunoglobulin production not only by the specified hybridoma cell line, but also by unspecified immunoglobulin producers. LDH from rabbit muscle enhanced IgM production of transcription-suppressed HB4C5 cells treated with actinomycin D. The IPSF effect of LDH was slightly weakened by sodium fluoride (translation inhibitor) treatment of HB4C5. Moreover, the amount of intracellular IgM of monensin-treated HB4C5 cells was obviously enhanced by LDH. This result means that the IPSF effect of LDH is irrelevant to the post-translation activity of target cells. It is expected from these findings that LDH from rabbit muscle accelerates the translation step to enhance immunoglobulin productivity. The immunoglobulin production stimulating activity of LDH was inhibited by colchicine, endocytosis inhibitor. This fact suggests that it is necessary for LDH to be taken by target cells for acting as IPSF. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
en : Cytotechnology 巻 42, 号 3, p. 133-143, 発行日 2003-09 |
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| 出版者 | ||||||||||
| 出版者 | Springer | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0920-9069 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA10678299 | |||||||||
| PubMed番号 | ||||||||||
| 識別子タイプ | PMID | |||||||||
| 関連識別子 | 19002935 | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1023/B:CYTO.0000015838.06536.de | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 日本十進分類法 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 464 | |||||||||
| 日本十進分類法 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 491.4 | |||||||||
